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A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Boekdetails

Serie
Methods in Molecular Biology
Ondertitel
Methods in Molecular Biology
Formaat
Hardback
Uitgever
Humana Press Inc.
Imprint
Humana Press Inc.
Auteur
Piet Herdewijn, Humana Press Inc., Humana Press Inc.
ISBN-13
9781588292339
Verschijningsdatum
2004-09-01
Editie
2005 ed.
Afmetingen
155 mm × 235 mm
Land van herkomst
US

Hardback

Oligonucleotide Synthesis: Methods and Applications

door Piet Herdewijn

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