This detailed new edition reflects the rapid evolution of Leptospira research over the past decade, particularly in genetics, genomics, imaging, and computational analysis. The book provides practical guidance for the manipulation of Leptospira spp. and for the investigation of leptospiral pathogenesis using state-of-the-art experimental approaches by introducing alternative media for direct isolation from infected hosts and for better mimicking host-associated conditions and by exploring gene editing tools, imaging-based approaches, and host-pathogen interactions, among other subject areas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Leptospira spp.: Methods and Protocols, Second Edition serves both established investigators and newcomers to the field, facilitating rigorous and innovative research on these fastidious yet fascinating organisms.