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A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E.

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Boekdetails

Serie
Methods in Molecular Biology
Ondertitel
Methods in Molecular Biology
Formaat
Paperback / softback
Uitgever
Humana Press Inc.
Imprint
Humana Press Inc.
Auteur
Nicola Casali || Andrew Preston, Humana Press Inc., Humana Press Inc.
ISBN-13
9781617373916
Verschijningsdatum
2010-11-10
Editie
1st ed. Softcover of orig. ed. 2003
Afmetingen
155 mm × 235 mm
Land van herkomst
NL

Paperback / softback

E. coli Plasmid Vectors: Methods and Applications

door Nicola Casali || Andrew Preston

€ 165,99
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